The Puppy Biome Project – Canine Microbiome
The Puppy Biome
Seeking Breeding Dogs for Gut Microbiome Longitudinal Study
It Takes Guts To Be Healthy
The Puppy Biome Project is one of the most extensive prospective study ever undertaken in veterinary gut microbiome research, gathering information on more than 2,000 breeding dogs and their puppies during their lives.

The study’s purpose is to identify the nutritional, environmental, lifestyle and genetic risk factors influencing gut microbiome acquisition in puppies with life long implication to their health. The data collected will inform veterinary medical research for decades to come.
In humans, it is thought that the mothers play a significant role in influencing the development of gut microbiome in their offspring which in turn affects their health with potential life-long implications.
The infant microbiome is said to play an essential role in human health and its acquisition is determined by mom-baby transfer of microbiota. This process can be impacted by several factors including caesarean surgery, emergency medical intervention, the use of antibiotics, and formula feeding. All have been linked to increased risks of disease.
We are launching a large scale longitudinal study to examine the gut microbiome of breeding dogs and puppies to explore the relationship that exists with their health. We aim to investigate preventive and restorative strategies to optimise puppy microbiota development, and highlight where research is needed to advance this field and improve the health of future generations.
The study aims to map the gut microbiome population of:

Breeding bitches on at least 3 occasions:

Gut microbiomes analysis will be matched to nutrition, life style, medical history, and medications as well as puppy delivery method (regular whelping, caesarian or emergency medical intervention).

Puppies (progeny of the above breeding females) to evaluate:

Next stage of this study will focus on:

How the gut microbiome can be manipulated around pregnancy to reduce chance of dystocia and and improve health outcomes for both dams and their offspring.
We expect this longitudinal study that will span 4-6 years and although mainly funded by Orivet and government research and development grants. We also seek the help of health-conscientious breeders and new puppy owners with partial funding of this study for the better health and wellbeing of pets anywhere.

Participation cost for each breeding bitch AUD$390 / USD$275 / £220 

Each dam will receive:

Participation cost for each Puppy to join AUD$290 / USD$205 / £163

Each puppy will receive:

Participation is easy and involves completing an online health questionnaire and sending dog’s fecal samples (collection kit and instructions provided) at various stages of the study (as described above).

If you’re interested, please fill out the form below. Feel free to pass it along, too.